Microgiobulinin Urine : Not Suitable for Assessing Renal TubularFunction

0.72x + 8.35 and correlation coefficient r = 0.999. This means that there is absolutely no indication of a different affinity of inhibitor to substrate, or of a greater degree of dissociation of the enzyme-inhibitor complex. In fact, the gradual increase in the reaction rate after 10 mm, as observed by Huang and Tietz in their assay in the presence ofinhibitor, might be a reflection of the lower inhibitor concentration they used. Nevertheless, the introduction of a linear standard curve by Huang and Tietz certainly adds to the attractiveness of the method of isoamylase determination by selective inhibition.